ROS Mediated the Effects of Extracellular Calcium on Staurosporine-induced Cell Death in PC12 Cells
نویسندگان
چکیده
Recently we showed that staurosporine uses the extracellular calcium ions to affect on neurite outgrowth in PC12 cells. However, these mechanisms during staurosporine-induced cell death are still unclear. In this study, we investigated the relation between of reactive oxygen species (ROS) and extracellular calcium entry during staurosporine-induced cell death in PC12 cells. The results showed that decrease or increase of extracellular calcium ions resulted in correspondingly significant increase in intracellular calcium concentration ([Ca2+]i). At high extracellular Ca2+ concentration (0.5-0.7 mM) increase in intracellular calcium concentration ([Ca2+]i), cells which was not prevented in the presence of IDB and GSH/EE, indicating its independence on ROS generation in treated cells. Another side, at low extracellular Ca2+ (0.-0.3 mM) was prevented in the presence of IDB and GSH/EE, indicating its dependence on ROS generation in treated cells. Thus, endogenous ROS generation and the rise in intracellular calcium by reducing extracellular calcium entry are important inter-players in staurosporine-induced cell death.
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